TRI Reagent® MRC

RNA isolation / purification Cells - primary human osteoblasts

Experiment
RNA isolation / purification Cells - primary human osteoblasts
Product
TRI Reagent® MRC from Molecular Research Center, Inc.
Manufacturer
Molecular Research Center, Inc.

Protocol tips

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA.

- The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

Total RNA was isolated using TRI Reagent (Molecular Research Center, Inc.).

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Reviews

TRI Reagent® MRC from Molecular Research Center, Inc. has not yet been reviewed for this experiment

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human osteoblasts using TRI Reagent® MRC from Molecular Research Center, Inc..

Paper title
Substrate Microarchitecture Shapes the Paracrine Crosstalk of Stem Cells with Endothelial Cells and Osteoblasts
Full paper
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Manufacturer protocol

Download the product protocol from Molecular Research Center, Inc. for TRI Reagent® MRC below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary human osteoblasts using TRI Reagent® MRC from Molecular Research Center, Inc.. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

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