RNeasy Plus Mini Kit

RNA isolation / purification Cells - primary human osteoblasts - rheumatoid arthritis

Experiment
RNA isolation / purification Cells - primary human osteoblasts - rheumatoid arthritis
Product
RNeasy Plus Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

At the end of the experiment total RNA was extracted using either the TRI Reagent (Sigma- Aldrich) for osteoblasts or the RNeasy Plus Mini Kit (Qiagen) for the endothelial cells.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human osteoblasts - rheumatoid arthritis using RNeasy Plus Mini Kit from Qiagen.

Paper title
Bones in human CYP26B1 deficiency and rats with hypervitaminosis A phenocopy overexpression
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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