RNeasy Mini Kit

RNA isolation / purification Cells - primary human placental epithelial cells

5/5 - 1 Review - 100% recommends this

Experiment

RNA isolation / purification Cells - primary human placental epithelial cells

Product

RNeasy Mini Kit from Qiagen

Manufacturer

Qiagen

Buy product Write review

Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was extracted from HPECs and HUVEC as previously described using a rapid RNA isolation system (Qiagen RNeasy, Courtaboeuf, France). Reverse transcription was performed on 1 μg total RNA with Superscript II-RnaseH reverse transcriptase (Invitrogen).

Full paper Login or join for free to view the full paper.

Reviews

Scientist

Review date

2021-05-17

Protocol

Standard

Product rating

Would recommend?

Yes

Rate review

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Comment View 1 comment

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human placental epithelial cells using RNeasy Mini Kit from Qiagen.

Paper title

Molecular Characterization of EG-VEGF-mediated Angiogenesis: Differential Effects on Microvascular and Macrovascular Endothelial Cells

Full paper

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary human placental epithelial cells using RNeasy Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

Outsource experiment