TriPure Isolation Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Do not wash cells before adding reagent (suspension cells).	- For low contamination rate carefully remove the upper aqueous phase. - Adding too little reagent can lead to contamination with DNA.

Upstream tips
- Do not wash cells before adding reagent (suspension cells).
Protocol tips
- For low contamination rate carefully remove the upper aqueous phase. - Adding too little reagent can lead to contamination with DNA.

Publication protocol

Total RNA was isolated from cultured HPAECs by using TriPure® Isolation Reagent (Roche, Indianapolis, USA). Integrity of the extracted RNA was confirmed with Bioanalizer (Agilent, Palo Alto, CA, USA).

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Papers

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TriPure Isolation Reagent below.

Download manufacturer protocol

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