TRIzol Reagent

RNA isolation / purification Cells - primary human pulmonary artery endothelial cells

Experiment
RNA isolation / purification Cells - primary human pulmonary artery endothelial cells
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

RNA isolated from HPAEC and HLMVEC with TRIZOL was transcribed using M-MLV reverse transcriptase and oligo dT.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human pulmonary artery endothelial cells using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Protein Phosphatase 2A Activity Is Required for Functional Adherent Junctions in Endothelial Cells
Full paper
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download PDF Download manufacturer protocol

Videos

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