mirVana™ miRNA Isolation Kit, with phenol

RNA isolation / purification Cells - primary human pulmonary artery smooth muscle cells

Experiment
RNA isolation / purification Cells - primary human pulmonary artery smooth muscle cells
Product
mirVana™ miRNA Isolation Kit, with phenol from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For RNA to dissolve better and to yeild high levels, preheat elution buffer at 95C.

- Addition of β-mercaptoethanol in lysis buffer can be skipped because the phenol will do the job of nuclease inactivation.

- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step.
Downstream tips
- Do vortex for 2-plus minutes during Acid-Phenol:Chloroform extraction step. This will facilitate the removal of tightly bound proteins typically associated with RNA.

Publication protocol

The mirVana miRNA Isolation Kit was used to extract and purify large RNA and small (micro) RNA that were enriched from HPASMC monolayer lysates according to the manufacturer’s protocol (Life Technologies). Following organic extraction and purification procedures, both large and small RNA fractions were eluted in separate vials, and RNA concentration and purity were determined by ultraviolet absorption using a spectrophotometer (BioTek, Winooski, VT).

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human pulmonary artery smooth muscle cells using mirVana™ miRNA Isolation Kit, with phenol from Thermo Fisher Scientific.

Paper title
Peroxisome proliferator-activated receptor-γ enhances human pulmonary artery smooth muscle cell apoptosis through microRNA-21 and programmed cell death 4
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for mirVana™ miRNA Isolation Kit, with phenol below.

Download PDF Download manufacturer protocol

Videos

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