Lipofectamine® 2000 Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

Transient transfections
The cells were plated at a density of 1 × 105 cells/well in a 24-well plate. After 12–24 h, the cells were cotransfected with 0.6 μg of the expression vector plasmids, 0.18 μg of the promoter reporter plasmids, and 0.02 μg of the pRL-TK plasmids using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. After 5 h of transfection, the cells were washed and allowed to recover overnight in fresh medium supplemented with 1% FBS for 48 h. Serum-starved cells were used for the assay.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® 2000 Transfection Reagent below.

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