TRIzol™ Plus RNA Purification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
RAECs were incubated in endothelial basal medium, TNF-α conditioned medium (10 ng/ml) or Slit2 conditioned medium (100 ng/ml) in a humidified incubator containing 5% CO2 at 37°C for 48 h.	Total cellular RNA was extracted using the TRIzol® Plus Purification kit (Thermo Fisher Scientific, Inc.), according to the manufacture's protocol.

Upstream tips
RAECs were incubated in endothelial basal medium, TNF-α conditioned medium (10 ng/ml) or Slit2 conditioned medium (100 ng/ml) in a humidified incubator containing 5% CO2 at 37°C for 48 h.
Protocol tips
Total cellular RNA was extracted using the TRIzol® Plus Purification kit (Thermo Fisher Scientific, Inc.), according to the manufacture's protocol.

Publication protocol

RAECs were incubated in endothelial basal medium, TNF-α conditioned medium (10 ng/ml) or Slit2 conditioned medium (100 ng/ml) in a humidified incubator containing 5% CO2 at 37°C for 48 h. Total cellular RNA was extracted using the TRIzol® Plus Purification kit (Thermo Fisher Scientific, Inc.), according to the manufacture's protocol.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol™ Plus RNA Purification Kit below.

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