TRIzol™ Plus RNA Purification Kit

RNA isolation / purification Cells - primary rabbit aortic endothelial cells

Experiment
RNA isolation / purification Cells - primary rabbit aortic endothelial cells
Product
TRIzol™ Plus RNA Purification Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
RAECs were incubated in endothelial basal medium, TNF-α conditioned medium (10 ng/ml) or Slit2 conditioned medium (100 ng/ml) in a humidified incubator containing 5% CO2 at 37°C for 48 h.
Protocol tips
Total cellular RNA was extracted using the TRIzol® Plus Purification kit (Thermo Fisher Scientific, Inc.), according to the manufacture's protocol.

Publication protocol

RAECs were incubated in endothelial basal medium, TNF-α conditioned medium (10 ng/ml) or Slit2 conditioned medium (100 ng/ml) in a humidified incubator containing 5% CO2 at 37°C for 48 h. Total cellular RNA was extracted using the TRIzol® Plus Purification kit (Thermo Fisher Scientific, Inc.), according to the manufacture's protocol.

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary rabbit aortic endothelial cells using TRIzol™ Plus RNA Purification Kit from Thermo Fisher Scientific.

Paper title
Slit2 suppresses endothelial cell proliferation and migration by inhibiting the VEGF-Notch signaling pathway
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol™ Plus RNA Purification Kit below.

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