TRIzol Reagent

RNA isolation / purification Cells - primary rat astrocytes

Experiment
RNA isolation / purification Cells - primary rat astrocytes
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

RNA was extracted using the TRIzol reagent following the directions of the manufacturer. Total RNA concentration and its purity were determined by spectrophotometry. Electrophoresis in 1.5% agarose gels was used to ensure good mRNA integrity. Levels of mRNA were determined by real-time reverse transcription polymerase chain reaction by the Functional Genomics Core Facility (Department of Environmental and Occupational Health Sciences, University of Washington) using TaqMan reverse transcription kits and an ABI 7900 HT Sequence Detection System (Applied Biosystems). β-Actin was used as the reference gene in all of the experiments.

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary rat astrocytes using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Retinoic Acid Isomers Up-Regulate ATP Binding Cassette A1 and G1 and Cholesterol Efflux in Rat Astrocytes: Implications for Their Therapeutic and Teratogenic Effects
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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