TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

RNA was extracted using the TRIzol reagent following the directions of the manufacturer. Total RNA concentration and its purity were determined by spectrophotometry. Electrophoresis in 1.5% agarose gels was used to ensure good mRNA integrity. Levels of mRNA were determined by real-time reverse transcription polymerase chain reaction by the Functional Genomics Core Facility (Department of Environmental and Occupational Health Sciences, University of Washington) using TaqMan reverse transcription kits and an ABI 7900 HT Sequence Detection System (Applied Biosystems). β-Actin was used as the reference gene in all of the experiments.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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