RNeasy Mini Kit

RNA isolation / purification Cells - primary rat astrocytes

Experiment
RNA isolation / purification Cells - primary rat astrocytes
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Tissue was first homogenized on ice using a mortar and pestle followed by passage through a QIAshredder column (Qiagen). Cells were scraped and sonicated on ice for 15–20 s at 25% power before of RNA purification was carried out using RNeasy Mini or Micro Kits followed by DNase I treatment (Qiagen) to remove any genomic DNA contaminants.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary rat astrocytes using RNeasy Mini Kit from Qiagen.

Paper title
Rhythmic expression of cytochrome P450 epoxygenases CYP4x1 and CYP2c11 in the rat brain and vasculature
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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