Lipofectamine® 2000 Transfection Reagent

DNA transfection Mammalian cells - Immortalized cell lines 293T

Experiment
DNA transfection Mammalian cells - Immortalized cell lines 293T
Product
Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

26. We use cationic lipid-based transfection reagents (Felgner and Ringold, 1989), Lipofectamine® 2000, Lipofectamine® 3000, and non-liposomal FuGENE® HD formulation to transiently transfect the 293FT cells with 2.5 μg of AtGORK plasmid obtained in step 10 according to the respective manufacturer's instructions (Table ​(Table1).1). ♦ CRITICAL STEP Equilibrate the transfection reagents to room temperature before use and mix by inverting or through a brief vortex. • OPTIONAL We recommend the use of OPTI-MEM® I (1X) Reduced Serum Medium as diluent in all transfection steps. Alternatively, serum-free DMEM (1X) + GlutaMAX™-I medium can be used for the transfection protocol. ❖ Troubleshooting (Table ​(Table22).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® 2000 Transfection Reagent below.

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