|Do not add antibiotics to the Transfection Medium
26. We use cationic lipid-based transfection reagents (Felgner and Ringold, 1989), Lipofectamine® 2000, Lipofectamine® 3000, and non-liposomal FuGENE® HD formulation to transiently transfect the 293FT cells with 2.5 μg of AtGORK plasmid obtained in step 10 according to the respective manufacturer's instructions (Table (Table1).1). ♦ CRITICAL STEP Equilibrate the transfection reagents to room temperature before use and mix by inverting or through a brief vortex. • OPTIONAL We recommend the use of OPTI-MEM® I (1X) Reduced Serum Medium as diluent in all transfection steps. Alternatively, serum-free DMEM (1X) + GlutaMAX™-I medium can be used for the transfection protocol. ❖ Troubleshooting (Table (Table22). Full paper
or join for free
to view the full paper.
Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Immortalized cell lines 293T using Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific.
Download the product protocol from Thermo Fisher Scientific for Lipofectamine® 2000 Transfection Reagent below.
Download manufacturer protocol
Check out videos that might be relevant for performing DNA transfection Mammalian cells - Immortalized cell lines 293T using Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.