Absolutely Total RNA Purification Kits

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Check your lysate, the viscosity should be low, high viscosity can result in low RNA yield and high DNA contamination. Perform extra vortexing, pipetting or add more lysis buffer. - Warm the elution buffer to 60`C for higher RNA yields.

Protocol tips
- Check your lysate, the viscosity should be low, high viscosity can result in low RNA yield and high DNA contamination. Perform extra vortexing, pipetting or add more lysis buffer. - Warm the elution buffer to 60`C for higher RNA yields.

Publication protocol

Total RNA was extracted from fibroblasts using a commercially available kit (Absolutely RNA Miniprep Kit, Agilent Technologies), according to the manufacturer's instructions. The quantification of RNA was performed using a NanoVue Spectrophotometer (GE Healthcare) by analyzing at A260/A280 and A260/A230.

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Papers

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Manufacturer protocol

Download the product protocol from Agilent Technologies for Absolutely Total RNA Purification Kits below.

Download manufacturer protocol

Videos

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