Absolutely Total RNA Purification Kits

RNA isolation / purification Cells - primary rat cardiac fibroblasts

Experiment
RNA isolation / purification Cells - primary rat cardiac fibroblasts
Product
Absolutely Total RNA Purification Kits from Agilent Technologies
Manufacturer
Agilent Technologies

Protocol tips

Protocol tips
- Check your lysate, the viscosity should be low, high viscosity can result in low RNA yield and high DNA contamination. Perform extra vortexing, pipetting or add more lysis buffer.

- Warm the elution buffer to 60`C for higher RNA yields.

Publication protocol

Total RNA was extracted from fibroblasts using a commercially available kit (Absolutely RNA Miniprep Kit, Agilent Technologies), according to the manufacturer's instructions. The quantification of RNA was performed using a NanoVue Spectrophotometer (GE Healthcare) by analyzing at A260/A280 and A260/A230.

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Reviews

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary rat cardiac fibroblasts using Absolutely Total RNA Purification Kits from Agilent Technologies.

Paper title
Glycosides from Bertoni Possess Insulin-Mimetic and Antioxidant Activities in Rat Cardiac Fibroblasts
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Manufacturer protocol

Download the product protocol from Agilent Technologies for Absolutely Total RNA Purification Kits below.

Download PDF Download manufacturer protocol

Videos

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