E.Z.N.A.® Total RNA Kit I

RNA isolation / purification Cells - primary rat cardiac fibroblasts

Experiment
RNA isolation / purification Cells - primary rat cardiac fibroblasts
Product
E.Z.N.A.® Total RNA Kit I from Omega Bio Tek
Manufacturer
Omega Bio Tek

Protocol tips

Protocol tips
The following might help to increase RNA yield

- Heat the DEPC Water to 70°C before adding to the column. - Increase the incubation time to 5 minutes.

- Increase the elution volume. - Repeat the elution step with fresh DEPC Water (this may increase the yield, but decrease the concentration).

- Repeat the elution step using the eluate from the first elution (this may increase yield while maintaining elution volume).

Publication protocol

Total RNA was extracted from cultured cardiac fibroblast cells using an EZNA Total RNA Kit (Omega Bio-Tek, USA), and cDNA was generated using a PrimeScript RT Reagent Kit with a gDNA Eraser (TaKaRa, Otsu, Japan).

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary rat cardiac fibroblasts using E.Z.N.A.® Total RNA Kit I from Omega Bio Tek.

Paper title
Tripartite Motif Protein 72 Regulates the Proliferation and Migration of Rat Cardiac Fibroblasts via the Transforming Growth Factor-β Signaling Pathway.
Full paper
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Manufacturer protocol

Download the product protocol from Omega Bio Tek for E.Z.N.A.® Total RNA Kit I below.

Download PDF Download manufacturer protocol

Videos

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