E.Z.N.A.® Total RNA Kit I

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	The following might help to increase RNA yield - Heat the DEPC Water to 70°C before adding to the column. - Increase the incubation time to 5 minutes. - Increase the elution volume. - Repeat the elution step with fresh DEPC Water (this may increase the yield, but decrease the concentration). - Repeat the elution step using the eluate from the first elution (this may increase yield while maintaining elution volume).

Protocol tips

The following might help to increase RNA yield - Heat the DEPC Water to 70°C before adding to the column. - Increase the incubation time to 5 minutes. - Increase the elution volume. - Repeat the elution step with fresh DEPC Water (this may increase the yield, but decrease the concentration). - Repeat the elution step using the eluate from the first elution (this may increase yield while maintaining elution volume).

Publication protocol

Total RNA was extracted from cultured cardiac fibroblast cells using an EZNA Total RNA Kit (Omega Bio-Tek, USA), and cDNA was generated using a PrimeScript RT Reagent Kit with a gDNA Eraser (TaKaRa, Otsu, Japan).

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Discussion

Papers

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Manufacturer protocol

Download the product protocol from Omega Bio Tek for E.Z.N.A.® Total RNA Kit I below.

Download manufacturer protocol

Videos

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