AllPrep DNA/RNA Mini Kit

RNA isolation / purification Cells - primary rat cortical neurons

Experiment
RNA isolation / purification Cells - primary rat cortical neurons
Product
AllPrep DNA/RNA Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- Complete disruption of plasma membranes of cells and organelles is absolutely required to release all the nucleic acids contained in the sample.

- Different samples require different methods to achieve complete disruption.

- Incomplete disruption results in significantly reduced nucleic acid yields.

- Overloading the spin columns significantly reduces nucleic acid yields.
Protocol tips
- Homogenizing the material is necessary to redice the viscosity of the lysates caused by cell disruption.

- Incomplete homogenization results in inefficient binding of DNA and RNA and therefore significantly reduced yield and purity of nucleic acids.

- Excessive homogenization, on the other hand, results in shorter genomic DNA fragments.

- The centrifugation temperature should be 20–25ºC.
Warm the lysate to 37ºC before transferring it to the AllPrep DNA spin column.

Publication protocol

Total RNA and gDNA were purified from neuron cultures (Qiagen AllPrep).

Full paper   Login or join for free to view the full paper.

Reviews

AllPrep DNA/RNA Mini Kit from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary rat cortical neurons using AllPrep DNA/RNA Mini Kit from Qiagen.

Paper title
Activity-Dependent A-to-I RNA Editing in Rat Cortical Neurons
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for AllPrep DNA/RNA Mini Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary rat cortical neurons using AllPrep DNA/RNA Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms