NucleoSpin® RNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Aliquot rDNase and store at -20 °C.	Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

RNA was isolated with NucleoSpin®RNA II kit (Macherey-Nagel, Duren, Germany), according to manufacturer's protocol. The quantity and quality of the isolated total RNA was evaluated spectrophotometrically using ND-2000-Spectrophotometer NanoDrop 2000 with software for analysis of nucleic acids (Thermo Fisher Scientific, Wilmington, Delaware, USA).

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Discussion

Papers

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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® RNA below.

Videos

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