NucleoSpin® RNA

RNA isolation / purification Cells - primary rat dermal fibroblasts

Experiment
RNA isolation / purification Cells - primary rat dermal fibroblasts
Product
NucleoSpin® RNA from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

RNA was isolated with NucleoSpin®RNA II kit (Macherey-Nagel, Duren, Germany), according to manufacturer's protocol. The quantity and quality of the isolated total RNA was evaluated spectrophotometrically using ND-2000-Spectrophotometer NanoDrop 2000 with software for analysis of nucleic acids (Thermo Fisher Scientific, Wilmington, Delaware, USA).

Full paper   Login or join for free to view the full paper.

Reviews

NucleoSpin® RNA from Macherey Nagel has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary rat dermal fibroblasts using NucleoSpin® RNA from Macherey Nagel.

Paper title
Influence of cartilage interstitial fluid on gene expression in cruciate ligament fibroblasts
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® RNA below.

We haven't found the manufacturer protocol for this product yet.

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary rat dermal fibroblasts using NucleoSpin® RNA from Macherey Nagel. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms