bisBenzimide H 33342 trihydrochloride

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1 × 10^5 cells/well were seeded.	- Cells were treated with various concentrations (0–4 μM) of ChK for 24 h. - Hoechst 33342 in PBS for 10 min in the dark at 37 °C.

Upstream tips
- 1 × 10^5 cells/well were seeded.
Protocol tips
- Cells were treated with various concentrations (0–4 μM) of ChK for 24 h. - Hoechst 33342 in PBS for 10 min in the dark at 37 °C.

Publication protocol

OVCAR-3, A2780/CP70 and IOSE-364 cells were seeded in 24-well plates at 1 × 105 cells/well and incubated overnight. Cells were treated with various concentrations (0–4 μM) of ChK for 24 h. After treatment, cells were stained with 10 μg/mL Hoechst 33342 (Sigma, St. Louis, MO) in PBS for 10 min in the dark at 37 °C. Cell apoptosis was examined under a fluorescence microscope (ZEISS), and data were collected from three independent experiments.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Apoptosis assay cell type - OVCAR-3 using bisBenzimide H 33342 trihydrochloride from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for bisBenzimide H 33342 trihydrochloride below.

Videos

Check out videos that might be relevant for performing Apoptosis assay cell type - OVCAR-3 using bisBenzimide H 33342 trihydrochloride from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.