TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was isolated from HPASMC or cultured RPASMC (isolated from 4‐ and 9‐month‐old rats) by TRIzol (Invitrogen, Carlsbad, CA) as per manufacturer's instructions followed by the quantitative analysis of precursor (pre‐miR) or mature (miR) miRNA or mRNA by real‐time qRT‐PCR.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download manufacturer protocol

Videos

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