TRIzol Reagent

RNA isolation / purification Cells - primary rat pulmonary artery smooth muscle cells

Experiment
RNA isolation / purification Cells - primary rat pulmonary artery smooth muscle cells
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was isolated from HPASMC or cultured RPASMC (isolated from 4‐ and 9‐month‐old rats) by TRIzol (Invitrogen, Carlsbad, CA) as per manufacturer's instructions followed by the quantitative analysis of precursor (pre‐miR) or mature (miR) miRNA or mRNA by real‐time qRT‐PCR.

Full paper   Login or join for free to view the full paper.

Reviews

TRIzol Reagent from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary rat pulmonary artery smooth muscle cells using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Network of Micros Mediate Translational Repression of Bone Morphogenetic Protein Receptor‐2: Involvement in ‐Associated Pulmonary Vascular Remodeling
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary rat pulmonary artery smooth muscle cells using TRIzol Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms