E.Z.N.A.® Total RNA Kit I

RNA isolation / purification Cells - primary bovine umbilical vein endothelial cells

Experiment
RNA isolation / purification Cells - primary bovine umbilical vein endothelial cells
Product
E.Z.N.A.® Total RNA Kit I from Omega Bio Tek
Manufacturer
Omega Bio Tek

Protocol tips

Protocol tips
The following might help to increase RNA yield :

- Heat the DEPC Water to 70°C before adding to the column.

- Increase the incubation time to 5 minutes.

- Increase the elution volume

. - Repeat the elution step with fresh DEPC Water (this may increase the yield, but decrease the concentration).

- Repeat the elution step using the eluate from the first elution (this may increase yield while maintaining elution volume).

Publication protocol

Total RNA was isolated from BUVECs with the E.Z.N.A Total RNA Kit® (Omega Bio-Tek, Norcross, GA, USA), according to the manufacturer’s instructions. The samples were treated with ribonuclease-free deoxyribonuclease I (Turbo DNA-free Kit, Ambion, UK) to remove any genomic DNA, and the RNA was quantified with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific).

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary bovine umbilical vein endothelial cells using E.Z.N.A.® Total RNA Kit I from Omega Bio Tek.

Paper title
Differential intracellular calcium influx, nitric oxide production, ICAM-1 and IL8 expression in primary bovine endothelial cells exposed to nonesterified fatty acids
Full paper
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Manufacturer protocol

Download the product protocol from Omega Bio Tek for E.Z.N.A.® Total RNA Kit I below.

Download PDF Download manufacturer protocol

Videos

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