E.Z.N.A.® Total RNA Kit I

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	The following might help to increase RNA yield : - Heat the DEPC Water to 70°C before adding to the column. - Increase the incubation time to 5 minutes. - Increase the elution volume . - Repeat the elution step with fresh DEPC Water (this may increase the yield, but decrease the concentration). - Repeat the elution step using the eluate from the first elution (this may increase yield while maintaining elution volume).

Protocol tips

The following might help to increase RNA yield : - Heat the DEPC Water to 70°C before adding to the column. - Increase the incubation time to 5 minutes. - Increase the elution volume . - Repeat the elution step with fresh DEPC Water (this may increase the yield, but decrease the concentration). - Repeat the elution step using the eluate from the first elution (this may increase yield while maintaining elution volume).

Publication protocol

Total RNA was isolated from BUVECs with the E.Z.N.A Total RNA Kit® (Omega Bio-Tek, Norcross, GA, USA), according to the manufacturer’s instructions. The samples were treated with ribonuclease-free deoxyribonuclease I (Turbo DNA-free Kit, Ambion, UK) to remove any genomic DNA, and the RNA was quantified with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific).

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary bovine umbilical vein endothelial cells using E.Z.N.A.® Total RNA Kit I from Omega Bio Tek.

Manufacturer protocol

Download the product protocol from Omega Bio Tek for E.Z.N.A.® Total RNA Kit I below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary bovine umbilical vein endothelial cells using E.Z.N.A.® Total RNA Kit I from Omega Bio Tek. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.