TRI Reagent® Sigma

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA. - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA. - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

RNA isolation and reverse transcription: Total RNA from primary freshly isolated pig aortic endothelial cells was isolated with TriReagent (Sigma, St. Louis, USA) following the manufacturer’s protocol and further purified using RNAeasy MinElute Cleanup kit (Qiagen, Hilden, Germany). Quantity and purity of extracted RNA samples were analysed by spectrophotometry (NanoDrop 1000; ThermoFisher, Waltham, MA, USA) before using them to reverse transcription or storing them at −80°C for a later use. Isolated RNA samples were also analysed for integrity and genomic DNA contamination by gel electrophoresis prior to their use in reverse transcription.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

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