RNeasy Plus Mini Kit

RNA isolation / purification Cells - primary porcine airway epithelial cells

Experiment
RNA isolation / purification Cells - primary porcine airway epithelial cells
Product
RNeasy Plus Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was extracted from virus-infected or mock-infected cells using RNeasy plus mini kit (Qiagen, Valencia, CA) per manufacturer’s instruction. Genomic DNA was removed by gDNA eliminator spin column supplied in the kit. The purity of RNA was assessed by calculating the ratio of OD260/OD280.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary porcine airway epithelial cells using RNeasy Plus Mini Kit from Qiagen.

Paper title
Differential Induction of Type I and Type III Interferons by Swine and Human Origin H1N1 Influenza A Viruses in Porcine Airway Epithelial Cells
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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