TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Cells were collected in PBS from the insert using cell-scrapers on ice. After centrifugation at 4 °C, 5000 rpm for 5 min, the cell pellet was lysed using TRIzolTM reagent (Life Technologies, Rockville, MD, USA). RNA extraction was performed according to the TRIzolTM Reagent protocol. RNA concentrations were measured using Nanodrop (Thermofisher Scientific).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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