TRIzol Reagent

RNA isolation / purification Cells - primary mouse tracheal epithelial cells

Experiment
RNA isolation / purification Cells - primary mouse tracheal epithelial cells
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Cells were collected in PBS from the insert using cell-scrapers on ice. After centrifugation at 4 °C, 5000 rpm for 5 min, the cell pellet was lysed using TRIzolTM reagent (Life Technologies, Rockville, MD, USA). RNA extraction was performed according to the TRIzolTM Reagent protocol. RNA concentrations were measured using Nanodrop (Thermofisher Scientific).

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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