Aurum™ Total RNA Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit is designed to process upto 2x10^6 cells - Before using lysis solution, add 500 ul of β-mercaptoethanol (β-ME) to the solution for a final concentration of 1%.	- To perform efficient elution preheat the elution solution at 70C in water bath priorto the elution step. - If there is high genomic DNA contamination, increase DNAse I digestion time and use only the DNAse dilution solution provided in the kit	- For better downstream application add appropriate volume of 95-100% ethanol to the wash solutions before intial use. - If elute volume is >80ul, there is a possibility of ethanol contamination. To reduce this, add 1-3min of centrifugation time after the final wash step.

Upstream tips
- This kit is designed to process upto 2x10^6 cells - Before using lysis solution, add 500 ul of β-mercaptoethanol (β-ME) to the solution for a final concentration of 1%.
Protocol tips
- To perform efficient elution preheat the elution solution at 70C in water bath priorto the elution step. - If there is high genomic DNA contamination, increase DNAse I digestion time and use only the DNAse dilution solution provided in the kit
Downstream tips
- For better downstream application add appropriate volume of 95-100% ethanol to the wash solutions before intial use. - If elute volume is >80ul, there is a possibility of ethanol contamination. To reduce this, add 1-3min of centrifugation time after the final wash step.

Publication protocol

The AurumTM Total RNA Mini Kit (BIO-RAD) was used to obtain RNA for microarray assays, following the manufacturer instructions. RNA concentration was determined with a NanoDrop 2000 (THERMO
SCIENTIFIC) and integrity was confirmed with a bioanalyzer 2100 (AGILENT) using the kit RNA 6000 nano reagents (AGILENT).

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Manufacturer protocol

Download the product protocol from Bio-Rad Laboratories for Aurum™ Total RNA Mini Kit below.

Download manufacturer protocol

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