NucleoSpin® RNA

RNA isolation / purification Cells - primary mouse cortical neurons

Experiment
RNA isolation / purification Cells - primary mouse cortical neurons
Product
NucleoSpin® RNA from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

Total RNA was isolated from cultured cells using Nucleospin RNA II kit (Macherey-Nagel) according to the manufacturer’s instructions. As much as 5 μg of RNA, quantified using NanoDrop spectrophotometer (Nanodrop 2000, Thermo Scientific), were preserved in RNAstable (Biomatrica) and subsequently shipped to KAUST’s next-generation sequencing core lab where it was reconstituted in RNAse-free water. The integrity and level of degradation of the purified RNA was assessed with the Agilent BioAnalyzer 2100 (Agilent Technologies, Inc.). RIN value was above 8 for all the samples, on a scale from 1 (degraded) to 10 (intact).

Full paper   Login or join for free to view the full paper.

Reviews

NucleoSpin® RNA from Macherey Nagel has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary mouse cortical neurons using NucleoSpin® RNA from Macherey Nagel.

Paper title
-Lactate Regulates the Expression of Synaptic Plasticity and Neuroprotection Genes in Cortical Neurons: A Transcriptome Analysis
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® RNA below.

We haven't found the manufacturer protocol for this product yet.

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary mouse cortical neurons using NucleoSpin® RNA from Macherey Nagel. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms