NucleoSpin® RNA

RNA isolation / purification Cells - primary mouse cortical neurons

Experiment
RNA isolation / purification Cells - primary mouse cortical neurons
Product
NucleoSpin® RNA from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

Total RNA was isolated from cultured cells using Nucleospin RNA II kit (Macherey-Nagel) according to the manufacturer’s instructions. As much as 5 μg of RNA, quantified using NanoDrop spectrophotometer (Nanodrop 2000, Thermo Scientific), were preserved in RNAstable (Biomatrica) and subsequently shipped to KAUST’s next-generation sequencing core lab where it was reconstituted in RNAse-free water. The integrity and level of degradation of the purified RNA was assessed with the Agilent BioAnalyzer 2100 (Agilent Technologies, Inc.). RIN value was above 8 for all the samples, on a scale from 1 (degraded) to 10 (intact).

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary mouse cortical neurons using NucleoSpin® RNA from Macherey Nagel.

Paper title
-Lactate Regulates the Expression of Synaptic Plasticity and Neuroprotection Genes in Cortical Neurons: A Transcriptome Analysis
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Manufacturer protocol

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