miRNeasy Mini kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To capture a higher amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step.

Protocol tips
- To capture a higher amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step.

Publication protocol

Briefly, total RNA was extracted using the miRNeasy kit with on-column digestion of genomic DNA (QIAGEN, Valencia, CA) according to the manufacturer’s instructions. RNA concentration was measured using the NanoDrop 2000 Spectrophotometer (Thermo Scientific, Wilmington, DE) and Qubit Fluorometric Quantitation (Invitrogen, Carlsbad, CA). Ratios of A260/280 nm were between 1.97 and 2.08. RNA integrity was assessed using RNA Nano chips in an Agilent 2100 Bionalyzer (Agilent technologies, Santa Clara, CA). RNA integrity numbers (RIN) were between 7.5 and 8.4.

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Manufacturer protocol

Download the product protocol from Qiagen for miRNeasy Mini kit below.

Download manufacturer protocol

Videos

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