miRNeasy Mini kit

RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons

Experiment
RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons
Product
miRNeasy Mini kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To capture a higher amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step.

Publication protocol

Briefly, total RNA was extracted using the miRNeasy kit with on-column digestion of genomic DNA (QIAGEN, Valencia, CA) according to the manufacturer’s instructions. RNA concentration was measured using the NanoDrop 2000 Spectrophotometer (Thermo Scientific, Wilmington, DE) and Qubit Fluorometric Quantitation (Invitrogen, Carlsbad, CA). Ratios of A260/280 nm were between 1.97 and 2.08. RNA integrity was assessed using RNA Nano chips in an Agilent 2100 Bionalyzer (Agilent technologies, Santa Clara, CA). RNA integrity numbers (RIN) were between 7.5 and 8.4.

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Reviews

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons using miRNeasy Mini kit from Qiagen.

Paper title
Dorsal root ganglion transcriptome analysis following peripheral nerve injury in mice
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Manufacturer protocol

Download the product protocol from Qiagen for miRNeasy Mini kit below.

Download PDF Download manufacturer protocol

Videos

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