NucleoSpin® RNA
RNA isolation / purification Tissue - Rat Adipose
4/5 - 1 Review - 100% recommends this
Protocol tips
| Upstream tips |
|---|
| - Aliquot rDNase and store at -20 °C. |
| Protocol tips |
| - Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer. |
Publication protocol
Total RNA was isolated from the tissues using the TRIzol reagent (Life Technologies) and the NucleoSpin RNAII Kit according to the manufacturer's protocol. The RNA concentration was measured by spectrophotometry at 260 nm. RNA purity was determined by calculating ratios of absorbance at 260/280 nm and 260/230 nm. The quality of the total RNA was verified by assessing the integrity of 18S/28S ribosomal RNA via electrophoresis in 1% ethidium bromide-stained agarose gels.Full paper Login or join for free to view the full paper.
Reviews
- Efficient
- Instructions can be simplified
Discussion
Discussion
3 years ago
Author:
is it suitable for human adipose tissue biopsy samples.
The kit works good in human tissue biopsy samples even with minimum amount of tissue.
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
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