RNeasy Plus Micro Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- When processing <500 cells or <2 µg tissue, carrier RNA may be added to the lysate. Foaming can be reduced by adding Reagent DX at a final concentration of 0.5% (v/v) before disruption and homogenization. - Add 4 volumes of ethanol (96–100%) to Buffer RPE for a working solution. before homogenization

Upstream tips
- When processing <500 cells or <2 µg tissue, carrier RNA may be added to the lysate. Foaming can be reduced by adding Reagent DX at a final concentration of 0.5% (v/v) before disruption and homogenization. - Add 4 volumes of ethanol (96–100%) to Buffer RPE for a working solution. before homogenization

Publication protocol

Cells located in the medial and lateral portions of the arcuate nucleus were individually captured into the lids of 0.2 ml PCR tubes containing 25 μl of lysis buffer (RNeasy plus micro kit; Qiagen). The lysis buffer volume was increased by 350 μl and the samples vortexed for 30 s and frozen at −80°C until RNA extraction (RNeasy plus micro kit; Qiagen). The RNA content of each sample was determined using a Nanodrop ND-1000 spectrophotometer and standardized; only samples with 260/280 readings between 1.60 and 2.00 were used for analysis. The RNA from each sample was then reverse transcribed using a Superscript kit (Superscript VILO; Invitrogen).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Adipose using RNeasy Plus Micro Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Micro Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Rat Adipose using RNeasy Plus Micro Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.