Dynabeads™ mRNA Purification Kit

RNA isolation / purification Tissue - Rat Adrenal glands

Experiment

RNA isolation / purification Tissue - Rat Adrenal glands

Product

Dynabeads™ mRNA Purification Kit from Thermo Fisher Scientific

Manufacturer

Thermo Fisher Scientific

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Keep the Dynabeads™ Oligo (dT)25 in liquid suspension during storage and handling steps. - All common buffers for mRNA purification and isolation can be used with Dynabeads™ Oligo (dT)25.

Protocol tips
- Keep the Dynabeads™ Oligo (dT)25 in liquid suspension during storage and handling steps. - All common buffers for mRNA purification and isolation can be used with Dynabeads™ Oligo (dT)25.

Publication protocol

The mRNA from the samples of agrenal glands was extracted using Dynabeads mRNA Purification Kit (Ambion, USA). NEBNext mRNA Library Prep Reagent Set for Illumina (NEB, USA) was used to construct the cDNA libraries following the manufacturer’s protocol.

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Reviews

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Adrenal glands using Dynabeads™ mRNA Purification Kit from Thermo Fisher Scientific.

Paper title

Molecular determinants of the adrenal gland functioning related to stress-sensitive hypertension in ISIAH rats

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Dynabeads™ mRNA Purification Kit below.

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