TRI Reagent® Sigma

Protocol tips

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- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

Expression studies were performed on adrenals of 3 randomly chosen rats from each experimental day. From regenerating and control glands total RNA was extracted using TRI Reagent (Sigma) and purified on columns (RNeasy Mini Kit, Qiagen). The amount of total mRNA was determined by optical density at 260 nm and its purity was estimated by 260/280 nm absorption ratio (higher than 1.8) (NanoDrop spectrophotometer, Thermo Scientific).

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

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