NucleoSpin® miRNA

RNA isolation / purification Tissue - Rat Artery / Aorta

Experiment

RNA isolation / purification Tissue - Rat Artery / Aorta

Product

NucleoSpin® miRNA from Macherey Nagel

Manufacturer

Macherey Nagel

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Aliquot rDNase and store at -20 °C.	- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

The MCAs and the basilar arteries of two animals were pooled (approx. 10 mg tissue in total), and total RNA was purified using a NucleoSpin miRNA kit (Macherey-Nagel, Germany) as described by the manufacturer

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Artery / Aorta using NucleoSpin® miRNA from Macherey Nagel.

Paper title

Regulation of microRNAs miR-30a and miR-143 in cerebral vasculature after experimental subarachnoid hemorrhage in rats

Full paper

Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® miRNA below.

Download manufacturer protocol

Videos

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