Magnetic mRNA Isolation Kit

RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat

Experiment

RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat

Product

Magnetic mRNA Isolation Kit from New England BioLabs

Manufacturer

New England BioLabs

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- If the lysate viscous than normal. Pass these cell or tissue lysates several times through a 21-gauge needle. If the yield of mRNA is low Increase the ratio of beads to Lysis/Binding Buffer or add RNase-free Proteinase K to the sample lysate directly before the 10 minute binding incubation

Protocol tips
- If the lysate viscous than normal. Pass these cell or tissue lysates several times through a 21-gauge needle. If the yield of mRNA is low Increase the ratio of beads to Lysis/Binding Buffer or add RNase-free Proteinase K to the sample lysate directly before the 10 minute binding incubation

Publication protocol

The total mRNA was extracted using the PureZOL reagent (Bio-Rad) and Magnetic mRNA Isolation Kit (NEB). First strand cDNA was synthesized with Random Hexamer primers and SuperScript III Reverse Transcriptase (Life Technologies).

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Reviews

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat using Magnetic mRNA Isolation Kit from New England BioLabs.

Paper title

Nutrient sensing modulates malaria parasite virulence

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Manufacturer protocol

Download the product protocol from New England BioLabs for Magnetic mRNA Isolation Kit below.

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