Magnetic mRNA Isolation Kit

RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat

Experiment

RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat

Product

Magnetic mRNA Isolation Kit from New England BioLabs

Manufacturer

New England BioLabs

Buy product Write review

Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- If the lysate viscous than normal. Pass these cell or tissue lysates several times through a 21-gauge needle. If the yield of mRNA is low Increase the ratio of beads to Lysis/Binding Buffer or add RNase-free Proteinase K to the sample lysate directly before the 10 minute binding incubation

Protocol tips
- If the lysate viscous than normal. Pass these cell or tissue lysates several times through a 21-gauge needle. If the yield of mRNA is low Increase the ratio of beads to Lysis/Binding Buffer or add RNase-free Proteinase K to the sample lysate directly before the 10 minute binding incubation

Publication protocol

The total mRNA was extracted using the PureZOL reagent (Bio-Rad) and Magnetic mRNA Isolation Kit (NEB). First strand cDNA was synthesized with Random Hexamer primers and SuperScript III Reverse Transcriptase (Life Technologies).

Full paper Login or join for free to view the full paper.

Reviews

Magnetic mRNA Isolation Kit from New England BioLabs has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

5 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Comment View 2 comments

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Comment View 2 comments

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat using Magnetic mRNA Isolation Kit from New England BioLabs.

Paper title

Nutrient sensing modulates malaria parasite virulence

Full paper

Manufacturer protocol

Download the product protocol from New England BioLabs for Magnetic mRNA Isolation Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat using Magnetic mRNA Isolation Kit from New England BioLabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

Outsource experiment