TRIzol Reagent
RNA isolation / purification Tissue - Rat Bone marrow
Protocol tips
| Protocol tips |
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| - For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it. |
Publication protocol
Nasal epithelial tissue samples were disrupted and homogenized using a TissueRuptor (Qiagen) and RNA isolated using the miRNeasy kit (Qiagen). For the WBC, samples were homogenized in TRIzol (Life Technologies) and RNA isolated according to the standard TRIzol protocol. The BM samples were filtered through 70-μm nylon mesh filters (Fischer Scientific, Waltham, Massachusetts) to remove bone fragments. Filtered BM samples were then homogenized in TRIzol (Life Technologies) and RNA isolated according to the standard TRIzol protocol. Extracted RNA was quantified with a Nanodrop 1000 spectrophotometer (Thermo Scientific, Waltham, Massachusetts) and its integrity verified with a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, California) using both the Nano and Small RNA kits. The quality control incorporated RNA integrity number assessment and electrophoretogram peak assessment of small RNA molecules.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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Manufacturer protocol
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