Magnetic mRNA Isolation Kit

RNA isolation / purification Tissue - Rat Brainstem

Experiment
RNA isolation / purification Tissue - Rat Brainstem
Product
Magnetic mRNA Isolation Kit from New England BioLabs
Manufacturer
New England BioLabs

Protocol tips

Protocol tips
- If the lysate viscous than normal. Pass these cell or tissue lysates several times through a 21-gauge needle. If the yield of mRNA is low Increase the ratio of beads to Lysis/Binding Buffer or add RNase-free Proteinase K to the sample lysate directly before the 10 minute binding incubation

Publication protocol

RNA from the rat tissues was extracted using the Magnetic mRNA Isolation kit (No. S1550S; New England Biolabs, Inc.). cDNA was synthesized using 200 ng mRNA, random hexamer primers, and RevertAid Premium (Thermo Fisher Scientific). PCR amplification was performed on 0.2 ng single-stranded cDNA with 2U Thermus aquaticus (Taq) DNA polymerase (Axon). Cycling conditions were 94°C for 3 min, 94°C for 30 s, 60°C for 20 s, 72°C for 30 s for 32 cycles, respectively, and 72°C for 8 min. Controls were performed on the cloned olfactory and retinal ANO 2 splice variants (see “Molecular cloning of rat ANO 1 and ANO 2”). The bands were resolved by gel electrophoresis and verified by sequencing.

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Discussion

Discussion

1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

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Manufacturer protocol

Download the product protocol from New England BioLabs for Magnetic mRNA Isolation Kit below.

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