PicoPure™ RNA Isolation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Can be used when there is a very less amount of starting material

Upstream tips
- Can be used when there is a very less amount of starting material

Publication protocol

Arcturus Picopure RNA extraction buffer (Applied Biosystems, Carlsbad, CA) was used the day of microdissection for lysing each sample. The samples were centrifuged briefly and kept at -80°C until the RNA isolation. RNA isolation was performed within five weeks of microdissection. RNA was extracted from normal tissue and PD-induced lesions. Picoscale RNA was isolated using the PicoPure RNA Isolation Kit (Molecular Devices, Sunnyvale, CA), adapting the protocol for use with CapSure LCM Caps. The amount of isolated RNA (pmole/μl) was determined with a Qubit Instrument. Isolated RNA was analyzed for quality and concentration using a Bioanalyzer PicoChip (Agilent Technologies, Englewood, CO). Twenty (20) ng of total RNA was amplified and labeled using the Nugen WT-Ovation Pico RNA Amplification and Encore Biotin Module protocols.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PicoPure™ RNA Isolation Kit below.

Download manufacturer protocol

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