NucleoSpin® RNA
RNA isolation / purification Tissue - Rat Colon
Protocol tips
| Upstream tips |
|---|
| - Aliquot rDNase and store at -20 °C. |
| Protocol tips |
| Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer. |
Publication protocol
RNA extraction was performed on jejunum and colon samples as per manufacturer’s instructions (NucleoSpin RNA Isolation Kit, Macherey-Nagel). Briefly, tissue samples were homogenized in 250 μl and 500 μl of TRIzol® Reagent (Invitrogen), respectively. Samples were centrifuged for 15 min at 4 °C and the upper aqueous layer removed. Following a sequence of filtration steps, RNA-binding conditions were adjusted and DNA digestion performed. A series of washing steps was performed before highly pure mRNA was eluted in RNase-free water. Once eluted, RNA was stored at −80 °C.Full paper Login or join for free to view the full paper.
Reviews
NucleoSpin® RNA from Macherey Nagel has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Colon using NucleoSpin® RNA from Macherey Nagel.
Manufacturer protocol
Download the product protocol from Macherey Nagel for NucleoSpin® RNA below.
We haven't found the manufacturer protocol for this product yet.
Videos
Check out videos that might be relevant for performing RNA isolation / purification Tissue - Rat Colon using NucleoSpin® RNA from Macherey Nagel. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.
Outsource your experiment
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment