Chromous Genomic DNA isolation kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

From the pure culture pellet, genomic DNA was isolated using Chromous Genomic DNA isolation kit. The ∼1.5 kb 16S-rDNA fragment was amplified using high fidelity PCR polymerase and the PCR product was sequenced bi-directionally by Genetic Analyzer (ABI 3130 Genetic Analyzer) using the forward and reverse primer (Bacillus specific universal primer). The NCBI BLAST program was used to analyze the sequence data of the isolates and these were aligned using the “ClustalW Submission Form” (http://www.ebi.ac.uk/clustalw/) and analyzed by ClustalW24. Evolutionary distances between the sequences were calculated using the method of Jukes and Cantor [20] and the phylogenetic tree was prepared following the “neighbor joining” method [21].

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Manufacturer protocol

Download the product protocol from Chromous Biotech for Chromous Genomic DNA isolation kit below.

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