TRIzol Reagent
RNA isolation / purification Tissue - Rat Epididymis
Protocol tips
| Protocol tips |
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| - For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it. |
Publication protocol
Total RNA was extracted from the rat epididymal fat tissue using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) according to the instructions provided by the manufacturer. Total RNA was subjected to reverse transcription in a 20 µL reaction containing random primers and SuperscriptII enzyme (Invitrogen). Quantitative real-time PCR was performed with an ABI Prism 7700 Sequence Detection System using SYBR® Green PCR Master Mix Reagent Kit (Applied Biosystems, Foster City, CA, USA)Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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Manufacturer protocol
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