Isolate II RNA Mini Kit
RNA isolation / purification Tissue - Rat Gut
Publication protocol
Total cellular RNA was extracted from one centimetre tissue segments using an “Isolate RNA Mini Kit” (Bioline, Luckenwalde, Germany) following the manufacturer’s protocol. The contaminating genomic DNA was digested with DNase I (Invitrogen, Karlsruhe, Germany). RNA concentration was measured spectrophotometrically using an infinite M200 micro plate reader (Tecan, Mainz-Kastel, Germany).Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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Manufacturer protocol
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