NucleoSpin® Soil

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Buffers A3 and AW contain guanidine hydrochloride which can form highly reactive compounds when combined with bleach (sodium hypochlorite). so DO NOT add bleach or acidic solutions directly to the sample preparation waste.

Upstream tips
Buffers A3 and AW contain guanidine hydrochloride which can form highly reactive compounds when combined with bleach (sodium hypochlorite). so DO NOT add bleach or acidic solutions directly to the sample preparation waste.

Publication protocol

Each isolate was grown anaerobically on M17 and MRS agar for 48 h at 37 °C. A single colony was suspended in 1 mL Milli-Q water and centrifuged for 1 min at 12,000 g. Genomic DNA was isolated from the pellet using the NucleoSpin® Soil Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany), following the manufacturer's instructions. The concentration of the resultant genomic DNA was measured with a NanoDrop 2000 UV-Vis spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA, USA).

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Discussion

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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® Soil below.

Download manufacturer protocol

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