NucleoSpin® Soil

DNA isolation / purification Bacteria - Gram positive Lactobacillus

Experiment
DNA isolation / purification Bacteria - Gram positive Lactobacillus
Product
NucleoSpin® Soil from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
Buffers A3 and AW contain guanidine hydrochloride which can form highly reactive compounds when combined with bleach (sodium hypochlorite). so DO NOT add bleach or acidic solutions directly to the sample preparation waste.

Publication protocol

Each isolate was grown anaerobically on M17 and MRS agar for 48 h at 37 °C. A single colony was suspended in 1 mL Milli-Q water and centrifuged for 1 min at 12,000 g. Genomic DNA was isolated from the pellet using the NucleoSpin® Soil Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany), following the manufacturer's instructions. The concentration of the resultant genomic DNA was measured with a NanoDrop 2000 UV-Vis spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA, USA).

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® Soil below.

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