High Pure RNA Isolation Kit
RNA isolation / purification Tissue - Rat Liver
Protocol tips
| Upstream tips |
|---|
| - Be careful to create an RNase-free working environment |
| Protocol tips |
| - Always mix the sample tube well after addition of each reagent. |
Publication protocol
Total RNA was isolated from 5 mg liver tissue using High Pure RNA isolation kit (Roche, Germany). According to the handbook kit, the samples were treated with DNase I enzyme supported in the kit to avoid DNA contamination. Finally, Optical Density (A260/A280 and A260/A230) and concentration were measured using Nanophotometer 2000c (Thermo Science, USA).Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
Download the product protocol from Roche Lifesciences for High Pure RNA Isolation Kit below.
Download manufacturer protocol
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