PowerSoil® DNA isolation

DNA isolation / purification Bacteria - Gram positive Lactobacillus

Experiment
DNA isolation / purification Bacteria - Gram positive Lactobacillus
Product
PowerSoil® DNA isolation from Mobio
Manufacturer
Mobio

Protocol tips

Upstream tips
Do not use bleach to clean the inside of the PowerVac™ Manifold or to rinse the PowerVac™ Mini Spin Filter Adapters when attached to the manifold.

Publication protocol

In this method, an enzymatic lysis was conducted before the PowerSoil™ DNA Isolation Kit (MO BIO Laboratories, Inc., Carlsbad, CA) was used. Briefly, 50 µl of lysozyme (10 mg/ml, Sigma-Aldrich) was added to a 500 µl aliquot of bacterial cells followed by incubation for 1 hour at 37°C. The remainder of the DNA extraction was continued beginning with step 2 of the manufacturer's protocol.

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Lactobacillus using PowerSoil® DNA isolation from Mobio.

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Manufacturer protocol

Download the product protocol from Mobio for PowerSoil® DNA isolation below.

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Videos

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