GenElute™ Mammalian Total RNA Miniprep Kit

RNA isolation / purification Tissue - Rat Ovaries

Experiment

RNA isolation / purification Tissue - Rat Ovaries

Product

GenElute™ Mammalian Total RNA Miniprep Kit from Sigma-Aldrich

Manufacturer

Sigma-Aldrich

Buy product Write review

Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Samples require immediate and thorough disruption in the Lysis Solution/2-ME Mixture to prevent degradation of RNA.

Upstream tips
- Samples require immediate and thorough disruption in the Lysis Solution/2-ME Mixture to prevent degradation of RNA.

Publication protocol

total RNA was isolated according to the manufacturer's instructions using the RNeasy Mini Kit (Qiagen), AllPrep RNA/Protein kit, GenElute Mammalian Total RNA Miniprep Kit (Sigma- Aldrich), or TRIzol Reagent (Life Technologies), followed by further RNA purification with the RNeasy kit.

Full paper Login or join for free to view the full paper.

Reviews

GenElute™ Mammalian Total RNA Miniprep Kit from Sigma-Aldrich has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Comment View 2 comments

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Comment View 2 comments

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Ovaries using GenElute™ Mammalian Total RNA Miniprep Kit from Sigma-Aldrich.

Paper title

Simultaneous isolation of mRNA and native protein from minute samples of cells.

Full paper

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for GenElute™ Mammalian Total RNA Miniprep Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Rat Ovaries using GenElute™ Mammalian Total RNA Miniprep Kit from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

Outsource experiment