TriPure Isolation Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Process tissues immediately and freeze at -60c or below for Low RNA degradation		- Adding too little reagent can lead to contamination with DNA

Upstream tips
- Process tissues immediately and freeze at -60c or below for Low RNA degradation
Downstream tips
- Adding too little reagent can lead to contamination with DNA

Publication protocol

pancreatic tissues were perfused with 1 ml RNA-later as the RNA stabilization reagent (Qiagen, USA) by an insulin syringe. Tissues were subsequently cut into small pieces with sterile scissors. The tubes that contained pancreatic tissue and RNA-later were processed for extraction by using the RNX-plus solution, TriPure, and RNeasy Micro Kits (Qiagen, USA) according to the manufacturers’ instructions after either 30 min, overnight in 4ºC, or following storage at -80ºC for one, three or seven days in order to compare the effect of preservation time on RNA integrity. In all conditions, the livers were removed from 30 rats and used as control tissue in a comparison of RNA quality between pancreatic and liver RNAs.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TriPure Isolation Reagent below.

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